Hong Kong Finance

Feb 14 2018

ELISA Procedures

#elisa #procedures, #antibody #exploration, #indirect #elisa, #capture #elisa # ELISA Procedures Indirect ELISA Reagents and Equipment Phosphate buffered saline (PBS) tablet: 10 mM phosphate buffer, pH 7.4, 150 mM NaCl (Product No. P4417 ) and 0.1% sodium azide (Product No. S2002 ). Carbonate-Bicarbonate buffer capsule, pH 9.6 (Product No. C3041 ). Washing buffer (PBS-T): 10 mM phosphate buffer pH 7.4, 150 mM NaCl, 0.05% Tween 20 (Product No. P3563 ). Monoclonal primary antibody. Antibody controls: species- and isotype-matched, non-specific immunoglobulin (e.g. mouse myeloma proteins as a control for a mouse monoclonal primary antibody) Alkaline phosphatase-conjugated secondary antibody or peroxidase-conjugated secondary …